Annexin VII overexpression 293T lysate (whole cell) (ab94175)

Overview

  • Product nameAnnexin VII overexpression 293T lysate (whole cell)
  • Description
    Annexin VII overexpression lysate
  • General notesab94175 is a 293T cell transfected lysate in which Human Annexin VII has been transiently over-expressed using a pCMV-Annexin VII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Tissue specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Annexin VII overexpression 293T lysate (whole cell) images

  • ab94175 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Annexin VII antibody (ab88539) at 1/500 dilution

    Lane 1 : Annexin VII overexpression 293T lysate (whole cell) (ab94175)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Annexin VII overexpression 293T lysate (whole cell) (ab94175)

ab94175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"