Anti-ANP antibody - N-terminal (ab91250)

Overview

  • Product nameAnti-ANP antibody - N-terminal
    See all ANP primary antibodies
  • Description
    Rabbit polyclonal to ANP - N-terminal
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human ANP aa 30-56 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 4878

  • Positive control
    • Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab91250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
ELISA 1/1000.

Target

  • FunctionHormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Involvement in diseaseAtrial standstill 2
    Atrial fibrillation, familial, 6
  • Sequence similaritiesBelongs to the natriuretic peptide family.
  • Post-translational
    modifications
    Cleaved by CORIN upon secretion to produce the functional hormone.
    Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANF antibody
    • ANF_HUMAN antibody
    • ANP antibody
    • ATFB6 antibody
    • Atrial natriuretic factor antibody
    • Atrial natriuretic peptide antibody
    • Atriopeptin antibody
    • Cardiodilatin antibody
    • Cardiodilatin-related peptide antibody
    • Cardionatrin antibody
    • CDD ANF antibody
    • CDD antibody
    • CDD-ANF antibody
    • CDP antibody
    • Natriuretic peptide A antibody
    • Natriuretic peptide precursor A antibody
    • NPPA antibody
    • PND antibody
    • Prepronatriodilatin antibody
    see all

Anti-ANP antibody - N-terminal images

  • Anti-ANP antibody - N-terminal (ab91250) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 17 kDa
  • IHC image of ANP staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91250, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ANP antibody - N-terminal (ab91250)

ab91250 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Tissue lysate - whole (atrium and ventricle)
Loading amount 75 µg
Specification atrium and ventricle
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"