Overview

  • Product nameANP (NPPA) Rat ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.2 ng/ml
  • Range
    0.25 ng/ml - 4 ng/ml
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig
  • Product overview

    Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.

    An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Alternative names
    • ANF
    • ATFB6
    • Atrial natriuretic factor
    • Atrial natriuretic peptide
    • Atriopeptin
    • Cardionatrin
    • CDD ANF
    • Natriuretic peptide A
    • NPPA
    • PND
    • Prepronatriodilatin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

ANP (NPPA) Rat ELISA kit images

  • Representative Standard Curve using ab108797.

Protocols

References for ANP (NPPA) Rat ELISA kit (ab108797)

This product has been referenced in:
  • Rössger K  et al. Reward-based hypertension control by a synthetic brain-dopamine interface. Proc Natl Acad Sci U S A 110:18150-5 (2013). ELISA ; Human . Read more (PubMed: 24127594) »

See 1 Publication for this product

Product Wall



The lab has testd several plasma and serum samples, all at no dilution (1x). The value may be 0.3 ng/ml for human plasma/serum, but it may be higher or lower depending on the sample. Each sample is different and some optimization may be requi...

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Human plasma and serum has been tested with this kit. Rat EDTA plasma has also been tested, so human EDTA plasma should work with the kit as well.

Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Cou...

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Thank you for your patience with this reply.
We would recommend doing a serial dilution to see which protein concentration will fall within the standard curve. Take a look at our sample preparation guide for additional information. http://www.abca...

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The amount of ANP in the vial of standard for the lot you have is 20 nanograms. Please contact us if you have other questions about the assay.

Thank you very much for your inquiry. I am very sorry for the delay.

We tried to find a concrete value to propose for the extraction kit, however this is not possible.We recommend that each laboratory does optimize the conditions, dilutions f...

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Thank you for your enquiry. I am sorry it has taken some time to confirm this information for you.

As discussed on the telephone, I am sorry that asfar as we are aware, ab108797ANP Rat ELISA kithas never been tested with samples from horse. ...

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Thank you for contacting Abcam regarding this issue. It is okay to use heparin coated tubes and avoid having to use the 0.1M sodium citrate. I hope this helps. If there is anything else that I can help you with please let me know.

Thank you for your inquiry. The kit will work on EDTA samples. Please feel free to contact us if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"