The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 20 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
ab115734 at 5ug/ml staining Anterior Gradient 2 in Human colon tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115734 at 5ug/ml staining Anterior Gradient 2 in Human small intestine tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Anterior Gradient 2 antibody (ab115734)
Anti-Anterior Gradient 2 antibody (ab115734) at 0.01 µg/ml + Human duodenum lysate (in RIPA buffer) at 35 µg developed using the ECL technique
Predicted band size : 20 kDa
References for Anti-Anterior Gradient 2 antibody (ab115734)
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