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TQTYEEALYK SKTSNKPL, corresponding to internal sequence amino acids 55-72 of Human Anterior Gradient 2.
Our Abpromise guarantee covers the use of ab22208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/100 - 1/1000.|
|ICC/IF||1/200. PubMed: 19359471|
ab22208 staining Anterior Gradient 2 in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.