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Recombinant fusion protein (Human)
Our Abpromise guarantee covers the use of ab43043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
|ICC/IF||Use a concentration of 4 - 8 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
ab43043 staining cells from a human pancreatic cell line by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/350 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"