Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab43043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 4 - 8 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • Sequence similaritiesBelongs to the AGR family.
  • Cellular localizationSecreted. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HPC 8 antibody
    • HPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all

Anti-Anterior Gradient 2 antibody images

  • Immunohistochemistry of AGR2 in human tissues prepared from paraffin-embedded tissue blocks. Rabbit anti-AGR2 antibody ab43043 was used as primary antibody at a dilution range of 2 ug/ml to 4 ug/ml.
  • All lanes : Anti-Anterior Gradient 2 antibody (ab43043) at 2 µg/ml

    Lane 1 : HeLa cell extract
    Lane 2 : PC3 cell extract

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 20 kDa
  • ab43043 staining cells from a human pancreatic cell line by ICC/IF.  Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  The primary antibody was diluted 1/350 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary.

    See Abreview

References for Anti-Anterior Gradient 2 antibody (ab43043)

This product has been referenced in:
  • Ho ME  et al. Prostate cancer cell phenotypes based on AGR2 and CD10 expression. Mod Pathol : (2013). Read more (PubMed: 23348903) »
  • Dumartin L  et al. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. Cancer Res 71:7091-102 (2011). WB ; Human . Read more (PubMed: 21948970) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (pancreatic cancer cell line)
Specification pancreatic cancer cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Aug 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"