Anti-Anterior Gradient 2 antibody (ab58598)

Overview

  • Product name
    Anti-Anterior Gradient 2 antibody
    See all Anterior Gradient 2 primary antibodies
  • Description
    Goat polyclonal to Anterior Gradient 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Anterior Gradient 2 aa 28-39 (internal sequence).
    Sequence:

    C-GAKKDTKDSRPK

  • Positive control
    • WB: Human colon and human duodenum tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • Tissue specificity
    Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • Sequence similarities
    Belongs to the AGR family.
  • Cellular localization
    Secreted. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, protein disulphide isomerase family member antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • Epididymis secretory protein Li 116 antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HEL S 116 antibody
    • HPC 8 antibody
    • hPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all

Anti-Anterior Gradient 2 antibody images

  • Anti-Anterior Gradient 2 antibody (ab58598) at 0.01 µg/ml + Human colon tissue lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 20 kDa

    Incubated with the primary antibody for 1 hour.

  • Anti-Anterior Gradient 2 antibody (ab58598) at 0.01 µg/ml + Human duodenum tissue lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

References for Anti-Anterior Gradient 2 antibody (ab58598)

ab58598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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