Anti-Anthrax EF/Adenylyl cyclase antibody (ab21267)
Key features and details
- Rabbit polyclonal to Anthrax EF/Adenylyl cyclase
- Suitable for: ELISA
- Reacts with: Bacillus anthracis
- Isotype: IgG
Overview
-
Product name
Anti-Anthrax EF/Adenylyl cyclase antibody -
Description
Rabbit polyclonal to Anthrax EF/Adenylyl cyclase -
Host species
Rabbit -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Bacillus anthracis -
Immunogen
Synthetic peptide corresponding to Anthrax EF/Adenylyl cyclase (C terminal).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use a concentration of 1 µg/ml. Note: at this concentration ab21267 will detect 10 ng of free peptide.
|
Notes |
---|
ELISA
Use a concentration of 1 µg/ml. Note: at this concentration ab21267 will detect 10 ng of free peptide. |
Target
-
Relevance
Anthrax infection is initiated by inhalation, ingestion or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF) and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. EF is a calmodulin and Ca++ dependent adenylate cyclase responsible for the edema seen in the disease. It is thought to benefit the B. anthracis bacteria by inhibiting cells of the host immune system. -
Alternative names
- Adenylyl cyclase antibody
- Bacillus anthracis EF antibody
- Cya antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab21267 has been referenced in 1 publication.
- He L et al. Deficiency of FAM3D (Family With Sequence Similarity 3, Member D), A Novel Chemokine, Attenuates Neutrophil Recruitment and Ameliorates Abdominal Aortic Aneurysm Development. Arterioscler Thromb Vasc Biol 38:1616-1631 (2018). PubMed: 29853563