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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up is ideally in a volume of 100-500µl.
Up to 500µg of antibody can be purified in each run.
The method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized. Note: This method cannot be used with samples containing relatively dilute antibody in large volumes (e.g. tissue culture supernatant). For larger volumes, we recommend the use of Antibody TCS Purification Kit (3 purifications) (ab109207) or Antibody Serum Purification kit (3 purifications) (ab109209).
The components of ab102784 are fully compatible with our Antibody conjugation kits however they are not compatible with our GOLD Antibody conjugation kits. To purify antibodies for use with our Gold conjugation kits, please use our Gold antibody purification kit (ab204909)
ab102784 is not suitable for goat antibody pufication.
|Components||Identifier||3 tests||1 tests|
|10x Binding Buffer||1 unit||1 unit|
|Additional Collecting Tubes||4 units||1 unit|
|Elution Buffer||1 unit||1 unit|
|Neutralizer||1 vial||1 vial|
|Protein A resin||White cap||3 vials||1 vial|
|Spin Cartridge/ Collecting tube assembly||1 unit||1 unit|
|Wash Buffer||1 unit||1 unit|