Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Overview

  • Product nameAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)
    See all Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) reagents
  • Description
    Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)
  • Tested applicationsSuitable for: IHC-Pmore details
  • General notesPROTOCOL:
    1. Place slides in a Coplin jar containing 1x Antigen Retrieval Buffer; cover with a vented plastic wrap and place the jar in microwave and set high power to boil and set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
    2. Wash sections with hot tap water for 1 minute.
    3. Wash sections in buffer for 2x3 minutes.
    4. Block endogenous peroxidase as usual.
    5. Wash sections in buffer for 2x3 minutes.
    6. Block non-specific sites with a Blocking reagent as usual.
    7. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).
    8. Wash sections in buffer for 2x3 minutes
    9. Rest of the procedure is same as routinely performed in your laboratory.

Properties

  • RelevanceAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH=9.0) pretreatment.

Applications

Our Abpromise guarantee covers the use of ab93684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

This product has been referenced in:
  • Li Y  et al. Perinatal nicotine exposure increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: role of angiotensin II receptors. Stroke 43:2483-90 (2012). Read more (PubMed: 22738920) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Please find the recommended protocol to use with ab93684 attached.

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