Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Storage bufferpH: 9.00
    Constituents: 5% Tris, 1.5% 2-butoxyethanol, 5% Sodium EDTA
    Note: Buffer 100x concentrated with detergent.
  • Concentration information loading...
  • Research Areas
  • RelevanceAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH=9.0) pretreatment.

Applications

Our Abpromise guarantee covers the use of ab93684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

This product has been referenced in:
  • Li Y  et al. Perinatal nicotine exposure increases vulnerability of hypoxic-ischemic brain injury in neonatal rats: role of angiotensin II receptors. Stroke 43:2483-90 (2012). Read more (PubMed: 22738920) »

See 1 Publication for this product

Product Wall

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for calling Abcam earlier today.

Please find the recommended protocol to use with ab93684 attached.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"