Overview

  • Product nameAnti-Antithrombin III antibody
    See all Antithrombin III primary antibodies
  • Description
    Sheep polyclonal to Antithrombin III
  • Tested applicationsSuitable for: IHC-P, Immunodiffusion, Immunoelectrophoresis, Double Immunodiffusionmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat, DogDoes not react with: Rabbit, Goat, Chicken, Guinea pig, Cow, Pig
  • Immunogen

    Human Antithrombin III purified from plasma.

  • Positive control
    • Human intestine tissue
  • General notesReactivity against the sera of the listed species has been assessed by double diffusion using 10 ul of antisera against 1 ul of species sera.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 99% Glycine buffered saline, 0.1% EACA, 0.01% Benzamidine, 0.03% EDTA
  • Concentration information loading...
  • PurityDEAE-Chromatography
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Immunodiffusion Use at an assay dependent dilution. RID: 6 ul antiserum/cm2 gel vs. 5 ul neat -1:10 dilution human plasma.
Immunoelectrophoresis Use at an assay dependent dilution. 100 ul antiserum vs. 5 ul plasma gives single arc. Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Double Immunodiffusion Use at an assay dependent dilution. 10 ul antiserum vs. 5 ul plasma.

Target

  • FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificityFound in plasma.
  • Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Anti-Antithrombin III antibody images

  • ab124259, at 5µg/ml, staining Antithrombin III in formalin-fixed, paraffin-embedded Human intestine vessels tissue.

References for Anti-Antithrombin III antibody (ab124259)

ab124259 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124259.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"