Anti-Antithrombin III antibody [BDI205] (ab20933)

Overview

  • Product nameAnti-Antithrombin III antibody [BDI205]
    See all Antithrombin III primary antibodies
  • Description
    Mouse monoclonal [BDI205] to Antithrombin III
  • Tested applicationsSuitable for: WB, Blocking, ELISA, Dot blot, Purification, ICC/IF, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human, Baboon
    Does not react with: Rabbit, Cow, Dog
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • This antibody gave a positive signal in ICC/IF in HepG2 cell line. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM Sodium phosphate. pH 7.4
  • Concentration information loading...
  • Purification notesPurified from ascites by Gel filtration and anion exchange.
  • ClonalityMonoclonal
  • Clone numberBDI205
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
Blocking Use at an assay dependent concentration.
ELISA Use a concentration of 1 - 5 µg/ml.
Dot blot Use a concentration of 1 - 5 µg/ml.
Purification Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Target

  • FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificityFound in plasma.
  • Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANT3_HUMAN antibody
    • Antithrombin antibody
    • Antithrombin III antibody
    • Antithrombin-III antibody
    • AntithrombinIII antibody
    • AT 3 antibody
    • AT III antibody
    • AT3 antibody
    • AT3D antibody
    • ATIII antibody
    • Heparin cofactor I antibody
    • MGC22579 antibody
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
    • Serine cysteine proteinase inhibitor clade C member 1 antibody
    • Serine proteinase inhibitor clade C member 1 antibody
    • Serpin C1 antibody
    • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
    • SERPINC1 antibody
    • THPH7 antibody
    see all

Anti-Antithrombin III antibody [BDI205] images

  • IHC image of Antithrombin III staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20933, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab20933 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20933, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Antithrombin III antibody [BDI205] (ab20933)

This product has been referenced in:
  • Gore S  et al. Heparin surfaces: Impact of immobilization chemistry on hemocompatibility and protein adsorption. J Biomed Mater Res B Appl Biomater 102:1817-24 (2014). WB . Read more (PubMed: 24711209) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The immunogen used to develop ab20933, MAb to Antithrombin III, was purified full-length Human Antithrombin III. The epitope has not been mapped for this antibody, therefore we cannot guarantee that it will recognize you...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (CHO cells transfected with human Antithrombin III)
Total protein in input 150000 cells
Specification CHO cells transfected with human Antithrombin III
Immuno-precipitation step Protein G
Username

Ning Wang

Verified customer

Submitted Oct 31 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"