Anti-Antithrombin III antibody [EP5372] (ab126598)

Overview

  • Product name
    Anti-Antithrombin III antibody [EP5372]
    See all Antithrombin III primary antibodies
  • Description
    Rabbit monoclonal [EP5372] to Antithrombin III
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human plasma, Human ovary cancer, Human testis and fetal lung lysates. Human liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
    • Tissue specificity
      Found in plasma.
    • Involvement in disease
      Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
    • Sequence similarities
      Belongs to the serpin family.
    • Post-translational
      modifications
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANT3_HUMAN antibody
      • Antithrombin antibody
      • Antithrombin III antibody
      • Antithrombin-III antibody
      • AntithrombinIII antibody
      • AT 3 antibody
      • AT III antibody
      • AT3 antibody
      • AT3D antibody
      • ATIII antibody
      • Heparin cofactor I antibody
      • MGC22579 antibody
      • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1 antibody
      • Serine cysteine proteinase inhibitor clade C member 1 antibody
      • Serine proteinase inhibitor clade C member 1 antibody
      • Serpin C1 antibody
      • Serpin family C member 1 antibody
      • Serpin peptidase inhibitor clade C (antithrombin) member 1 antibody
      • SERPINC1 antibody
      • THPH7 antibody
      see all

    Images

    • All lanes : Anti-Antithrombin III antibody [EP5372] (ab126598) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human ovary cancer lysate
      Lane 3 : Human testis lysate
      Lane 4 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • ab126598, at 1/50 dilution, staining Antithrombin III in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Gillrie MR  et al. Thrombin Cleavage of Plasmodium falciparum Erythrocyte Membrane Protein 1 Inhibits Cytoadherence. MBio 7:N/A (2016). Read more (PubMed: 27624125) »
    • Wilson GJ  et al. GCN2 is required to increase fibroblast growth factor 21 and maintain hepatic triglyceride homeostasis during asparaginase treatment. Am J Physiol Endocrinol Metab 308:E283-93 (2015). Read more (PubMed: 25491724) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Lanes 1-4 in the western blot image on the datasheet http://www.abcam.com/antithrombin-iii-antibody-ep5372-ab126598.html are human plasma, ovary, testis, and fetal lung lysate, respectively. Thus, these were all tissue lysates rather than cell lysates....

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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