Antithrombin III overexpression 293T lysate (whole cell) (ab94043)

Overview

  • Product name
    Antithrombin III overexpression 293T lysate (whole cell)
  • General notes
    ab94043 is a 293T cell transfected lysate in which Human Antithrombin III has been transiently over-expressed using a pCMV-Antithrombin III plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Disease: Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. PTM: Phosphorylation sites are present in the extracelllular medium. Similarity: Belongs to the serpin family. Tissue specificity: Found in plasma.

Applications

Our Abpromise guarantee covers the use of ab94043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94043 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Antithrombin III antibody (ab66234) at 1/500 dilution

    Lane 1 : Antithrombin III overexpression 293T lysate (whole cell) (ab94043)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94043 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab94043.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up