Recombinant Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7717] to ANTXR2/CMG-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ANTXR2/CMG-2 antibody [EPR7717]
See all ANTXR2/CMG-2 primary antibodies -
Description
Rabbit monoclonal [EPR7717] to ANTXR2/CMG-2 -
Host species
Rabbit -
Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC for mouse.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ANTXR2/CMG-2 aa 400-500. The exact sequence is proprietary.
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Positive control
- WB: Human fetal heart, ECV304 and PC3 lysates, Mouse brain and heart lysate; IHC-P: Human colon tissue; ICC/IF: PC3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Please note: ab129004 seems to be not suitable for the use in IHC-P with mouse samples. |
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Please note: ab129004 seems to be not suitable for the use in IHC-P with mouse samples. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Necessary for cellular interactions with laminin and the extracellular matrix. -
Tissue specificity
Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen. -
Involvement in disease
Defects in ANTXR2 are the cause of infantile systemic hyalinosis (ISH) [MIM:236490]. This autosomal recessive syndrome is similar to JHF, but has an earlier onset and a more severe course. Symptoms appear at birth or within the first months of life, with painful, swollen joint contractures, osteopenia, osteoporosis and livid red hyperpigmentation over bony prominences. Patients develop multiple subcutaneous skin tumors and gingival hypertrophy. Hyaline deposits in multiple organs, recurrent infections and intractable diarrhea often lead to death within the first 2 years of life. Surviving children may suffer from severely reduced mobility due to joint contractures.
Defects in ANTXR2 are the cause of juvenile hyaline fibromatosis (JHF) [MIM:228600]. JHF is an autosomal recessive syndrome that is similar to ISH but takes a milder course. It is characterized by hyaline deposition in the dermis, multiple subcutaneous skin tumors and gingival hypertrophy, followed by progressive joint contractions, osteopenia and osteoporosis that may lead to a severe limitation of mobility. -
Sequence similarities
Belongs to the ATR family.
Contains 1 VWFA domain. -
Domain
Binding to PA seems to be effected through the VWA domain. -
Cellular localization
Secreted; Cell membrane. Expressed at the cell surface and Endoplasmic reticulum membrane. Expressed predominantly within the endoplasmic reticulum and not at the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 118429 Human
- Entrez Gene: 71914 Mouse
- Omim: 608041 Human
- SwissProt: P58335 Human
- SwissProt: Q6DFX2 Mouse
- Unigene: 162963 Human
- Unigene: 24842 Mouse
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Alternative names
- 2310046B19Rik antibody
- Anthrax toxin receptor 2 antibody
- ANTR2_HUMAN antibody
see all
Images
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All lanes : Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell from embryonic kidney transformed with large T antigen) transfected with an empty expression vector, whole cell lysate
Lane 2 : HEK-293T transfected with full length His-tagged Human ANTR1 expression vectors, whole cell lysate
Lane 3 : HEK-293T transfected with full length His-tagged Human ANTR2 expression vectors, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking and dilution buffer: 5% NFDM/TBST.
ab129004 doesn't recognize overexpressed ANTXR1.
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All lanes : Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-ANTXR2/CMG-2 antibody [EPR7717] (ab129004) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : ECV304 cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa -
ab129004, at 1/250 dilution, staining ANTXR2/CMG-2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab129004, at 1/250 dilution staining ANTXR2/CMG-2 in PC3 cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129004 has been referenced in 1 publication.
- Meng L et al. Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension. FASEB J 35:e21421 (2021). PubMed: 33749907