The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
FunctionHas a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina.
Tissue specificityExpressed in many tissues with much higher expression in retina. Isoform 1 and isoform 2 are expressed in adipose tissue, whereas isoform 1 only seems to be present in thymus, and isoform 2 only in testis.
Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
Post-translational modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
Cellular localizationCell membrane. Present on the surface of the cells and Cytoplasm. Either not translocated to the plasma membrane or below detection level.