Overview

  • Product nameAnti-AOC2 antibody
    See all AOC2 primary antibodies
  • Description
    Rabbit polyclonal to AOC2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within residues: AEDIPNTVTL GNRVGFLLRP YNFFDEDPSI FSPGSVYFEK GQDAGLCSIN , corresponding to internal sequence amino acids 684-733 of Human AOC2 (NP_033720).

  • Positive control
    • Fetal lung lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

  • FunctionHas a monoamine oxidase activity with substrate specificity for 2-phenylethylamine and tryptamine. May play a role in adipogenesis. May be a critical modulator of signal transmission in retina.
  • Tissue specificityExpressed in many tissues with much higher expression in retina. Isoform 1 and isoform 2 are expressed in adipose tissue, whereas isoform 1 only seems to be present in thymus, and isoform 2 only in testis.
  • Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
  • Post-translational
    modifications
    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
  • Cellular localizationCell membrane. Present on the surface of the cells and Cytoplasm. Either not translocated to the plasma membrane or below detection level.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amine oxidase [copper containing] antibody
    • Amine oxidase [copper-containing] antibody
    • Amine oxidase copper containing 2 (retina specific) antibody
    • Amine oxidase copper containing 2 antibody
    • AOC2 antibody
    • AOC2_HUMAN antibody
    • DAO2 antibody
    • RAO antibody
    • Retina specific copper amine oxidase antibody
    • Retina-specific copper amine oxidase antibody
    • Semicarbazide-sensitive amine oxidase antibody
    • SSAO antibody
    see all

Anti-AOC2 antibody images

  • Anti-AOC2 antibody (ab83734) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 84 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-AOC2 antibody (ab83734)

ab83734 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"