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Overview

  • Product nameAOS1+UBA2 protein
  • Expression hostBaculovirus

    • Properties

  • Purification notesThe Sumo E1 protein is a heterodimer consisting of human Aos 1 and Uba 2. Both the Aos 1 and Uba 2 are co-expressed in a baculovirus expression system, prepared from freshly extracted cell lysates, and purified to homogeneity. The final fraction of Aos 1 and Uba 2 is an affinity column pool.
  • Biological activityThe final fraction of enzyme contains two single polypeptide bands of 38 kDa and 80 kDa.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab3804 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
    Functional Studies
  • Application notesSumoylation assays are carried out in a final volume of 20 µl in reaction buffer (20 mM Hepes pH 7.5, 5mM MgCl2, 2mM ATP). We would recommend adding fresh 2mM ATP to be sure that sufficient energy is supplied.

    Uba 2 contains his-rich regions which might cross-react with antibodies against the 6x-His epitope tag.

    During western analysis, Uba 2 at 80 kDa might be shown.

    • Protein info

    • Alternative names
        Aos 1SAE ISAE II
        SAE1SAE2SUA1SUMO 1 activating enzyme subunit 1SUMO1 activating enzyme subunit 2Uba 2Ubiquitin like protein activating enzymeUbiquitin-like 1-activating enzyme E1AUbiquitin-like 1-activating enzyme E1BUBLE1AUBLE1B
      see all
  • RelevanceE1-SUMO protein is a heterodimer consisted of human AOS1 and UBA2. The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
  • Cellular localizationNuclear

    • AOS1+UBA2 protein images

    • Sumoylation assay
      Sumoylation assay

      Left Lane: Topo I protein (Topo I fragment: ab3828)

      Middle Lane: Sumo 1 sumoylated Topo I (Sumo 1: ab3801; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)

      Right Lane: Sumo 3 sumoylated Topo I (Sumo 3: ab3802; Topo I fragment: ab3828; EI: ab3804; EII: ab3803; Buffer: ab3827)

      Note: Topo I is S35-Met labeled.

    • - AOS1+UBA2 protein (ab3804)
      - AOS1+UBA2 protein (ab3804)

    References for AOS1+UBA2 protein (ab3804)

    ab3804 has not yet been referenced specifically in any publications.

    Publishing research using ab3804 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab3804 Submit a Question for ab3804

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    Votes: Highest First

    I asked about Ubc9 protein(ab3803), not Uba2 which is Ab3804. And I already know Uba2 is cross reactive with His antibody as it is in the data sheet. Let me know about Ubc9 after you check your catalog again. Thanks,

    Thank you for getting back to me. According to the details that I have Ubc9 protein (ab3803) is not epitope tagged. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"