AP Stability Conjugate Reagent (ab171536)


  • Product name
    AP Stability Conjugate Reagent
    See all ELISA buffer reagents
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s AP Stability Conjugate Reagent (ab171536) was developed specifically for Alkaline Phosphatase (AP) conjugates in solution. ab171536 prevents the loss of catalytic activity and maintains stability of proteins in solution. It allows conjugate storage at use concentration, resulting in stable conjugates that require no dilution. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios.

    Recommendations for Use of ab171536 AP Stability Conjugate Reagent

    • For optimal stability and performance, use ab171536 at 100% concentration
    • Dilute your AP conjugate to optimum working concentration in the AP Stability Conjugate Reagent solution
    • Use diluted conjugate working solution in your assay as normal
    • Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency
    • For added stability, add 10% glycerol to the AP conjugate / AP Stability Conjugate Reagent solution


  • Form
  • Storage instructions
    Store at room temperature.
  • Storage buffer
    Preservative: 0.02% 5-Bromo-5-nitro-1,3-dioxane
    Constituents: MES, 99% Proprietary component, 0.02% 2-methyl-4-isothiazolin-3-one
    Note: pH 5.5 - 6.5. Contains BSA
  • Concentration information loading...

Associated products


Our Abpromise guarantee covers the use of ab171536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Images

    • AP Stability Conjugate Reagent (ab171536) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.

      The studies were conducted using a monoclonal IgG-AP conjugate. Results of these analyses indicate superior performance of ab171536 compared to a common AP conjugate diluent. The monoclonal IgG-AP conjugate retained over 70% of its activity when stored in AP Stability Conjugate Reagent for 24 months at room temperature.


    ab171536 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We use a 1:3000 dilution of the conjugate in ab171536. We recommend diluting your AP conjugate at your normal use concentration first. You may consider trying a small accelerated study to determine whether or not you are getting the stability you need....

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    We recommend using ab171536 undiluted for maximum stabilization. However, in cases where you feel it may be necessary to dilute it (e.g., 1:1) please use ab171536 diluted in deionized water or 10 mM MES buffer, pH 6.0.
    NOTE: Phosphate buffers cause...

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