The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-Fr: 1/500 - 1/5000. depending on tissue and
IHC-P: 1/500 - 1/5000. depending on tissue and
WB: 1/2000 - 1/10000. depending on detection method. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSubunit of clathrin associated adaptor protein complex 1 that plays a role in protein sorting in the late Golgi/trans Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Adaptor protein complex 1 (AP1) is an heterotetramer composed of two large adaptins (gamma type subunit AP1G1 and beta type subunit AP1B1), a medium adaptin (mu type subunit AP1M1 or AP1M2) and a small adaptin (sigma type subunit AP1S1 or AP1S2 or AP1S3).
Cellular localizationCell Membrane and Cytoplasmic