Anti-AP1M1 antibody (ab230273)
Key features and details
- Rabbit polyclonal to AP1M1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP1M1 antibody
See all AP1M1 primary antibodies -
Description
Rabbit polyclonal to AP1M1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human AP1M1 aa 1-200.
Database link: Q9BXS5 -
Positive control
- WB: A-375, Jurkat, HeLa and HepG2 whole cell lysates. IHC-P: Human placenta and skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 49 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. -
Sequence similarities
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
Post-translational
modificationsPhosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 504310 Cow
- Entrez Gene: 8907 Human
- Entrez Gene: 11767 Mouse
- Entrez Gene: 306332 Rat
- Omim: 603535 Human
- SwissProt: Q2KJ81 Cow
- SwissProt: Q9BXS5 Human
- SwissProt: P35585 Mouse
see all -
Alternative names
- Adaptor protein complex AP-1 mu-1 subunit antibody
- Adaptor-related protein complex 1 mu-1 subunit antibody
- AP-1 complex subunit mu-1 antibody
see all
Images
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All lanes : Anti-AP1M1 antibody (ab230273) at 1/1000 dilution
Lane 1 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa -
Paraffin-embedded human placenta tissue stained for AP1M1 using ab230273 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human skin tissue stained for AP1M1 using ab230273 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230273 has been referenced in 1 publication.
- Kumari S et al. HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation. Life Sci N/A:N/A (2019). PubMed: 30953643