Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)

Overview

  • Product name
    Anti-AP2 alpha + beta antibody [A6/2/2]
    See all AP2 alpha + beta primary antibodies
  • Description
    Mouse monoclonal [A6/2/2] to AP2 alpha + beta
  • Specificity
    Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISA, IHC-P, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C-terminal peptide of AP-2 alpha (PNSHTDNNAKSSDKEEKHRK).

  • Positive control
    • Raji cells or breast carcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Phosphate buffered saline
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    A6/2/2
  • Myeloma
    NS0
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance
    The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. AP2 alpha is the only AP2 protein required for early morphogenesis of the lens vesicle. AP2 beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer binding protein 2 beta antibody
    • Activator protein 2 antibody
    • AP 2 antibody
    • AP 2alpha antibody
    • AP 2B antibody
    • AP-2 transcription factor antibody
    • AP2 B antibody
    • AP2-alpha antibody
    • AP2-beta antibody
    • AP2TF antibody
    • BOFS antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • TFAP2B antibody
    • Transcription factor AP2 alpha antibody
    • Transcription factor AP2 beta antibody
    see all

Images

  • ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Gee JM  et al. Immunohistochemical analysis reveals a tumour suppressor-like role for the transcription factor AP-2 in invasive breast cancer. J Pathol 189:514-20 (1999). Read more (PubMed: 10629551) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Hela)
Loading amount
30 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing
Username

Abcam user community

Verified customer

Submitted Jul 30 2008

Thank you for your enquiry. Raji cells or breast carcinomas should be a suitable positive control. I hope this information helps. Please do not hesitate to contact us if you need anything further.

The antibody A6/2/2 was raised against the peptide PNSHTDNNAKSSDKEEKHRK which is the C-terminus of AP-2alpha. As the antibody also recognises AP 2beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and ...

Read More

We always recommend that conditions for WB should be optimised by the end user. We suggest starting with a 1:1000 dilution.

This antibody recognises the transcription factor AP-2 and thus not the adaptor protein (AP-2).

We don't have any information on this antibody being used in EMSA, but as the immunogen was a C-terminal peptide chances are quite high it will work.

This antibody works in immunohistochemistry with paraffin embedded sections (fixative formal saline with calcium, 10 min microwave treatment required). However it has not been tested for whole mount IHC as you describe.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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