• Product name
  • Description
    Rabbit polyclonal to AP2 alpha
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 705-735 of Human AP2 alpha (NP_570603.2, NP_055018.2).

  • Positive control
    • 293 cell line lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab116289 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab116289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 108 kDa.


  • Function
    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
  • Tissue specificity
    Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells.
  • Sequence similarities
    Belongs to the adaptor complexes large subunit family.
  • Cellular localization
    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa coated vesicle protein A antibody
    • Adapter-related protein complex 2 alpha-1 subunit antibody
    • adapter-related protein complex 2 subunit alpha-1 antibody
    • Adaptin alpha A antibody
    • Adaptin alpha antibody
    • Adaptor protein complex AP 2 alpha 1 subunit antibody
    • adaptor protein complex AP 2 subunit alpha 1 antibody
    • Adaptor protein complex AP-2 subunit alpha-1 antibody
    • Adaptor protein complex AP2 alpha 1 subunit antibody
    • adaptor protein complex AP2 subunit alpha 1 antibody
    • Adaptor related protein complex 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP 2 alpha 1 subunit antibody
    • Adaptor related protein complex AP2 alpha 1 subunit antibody
    • ADTAA antibody
    • Alpha A adaptin antibody
    • Alpha adaptin A antibody
    • Alpha-adaptin A antibody
    • alpha1 adaptin antibody
    • Alpha1-adaptin antibody
    • AP 2 alpha antibody
    • AP 2 complex subunit alpha 1 antibody
    • AP-2 complex subunit alpha-1 antibody
    • AP2 complex subunit alpha 1 antibody
    • AP2-ALPHA antibody
    • Ap2a1 antibody
    • AP2A1_HUMAN antibody
    • CLAPA 1 antibody
    • CLAPA1 antibody
    • Clathrin adaptor complex AP2. alpha subunit antibody
    • Clathrin assembly protein complex 2 alpha A large chain antibody
    • Clathrin assembly protein complex 2 alpha-A large chain antibody
    • Clathrin associated / assembly / adaptor protein large alpha 1 antibody
    • clathrin associated assembly adaptor protein large alpha 1 antibody
    • clathrin associated/assembly/adaptor protein, large, alpha 1 antibody
    • Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody
    • Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
    see all


  • Anti-AP2 alpha antibody (ab116289) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 108 kDa


This product has been referenced in:
  • Zhao Z  et al. Central role for PICALM in amyloid-ß blood-brain barrier transcytosis and clearance. Nat Neurosci 18:978-87 (2015). Read more (PubMed: 26005850) »

See 1 Publication for this product

Customer reviews and Q&As

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Ab116289 was made from a region that is missing in isoform 2, so ab11326 may be better for your purposes.

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