Anti-AP-2 complex subunit alpha-1 antibody (ab189995)
Key features and details
- Goat polyclonal to AP-2 complex subunit alpha-1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AP-2 complex subunit alpha-1 antibody
See all AP-2 complex subunit alpha-1 primary antibodies -
Description
Goat polyclonal to AP-2 complex subunit alpha-1 -
Host species
Goat -
Specificity
ab189995 is expected to recognize reported isoform 1 (NP_055018.2) only. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig, Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to Human AP-2 complex subunit alpha-1 aa 700-800 (internal sequence) (Cysteine residue). NP_055018.2.
Database link: O95782 -
Positive control
- Human brain cortex tissue; HeLa cells, stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1; Mouse brain lysate and Human Frontal Cortex lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 0.1 µg/ml.
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WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 108 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.1 µg/ml. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 108 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. -
Tissue specificity
Isoform A expressed in forebrain, skeletal muscle, spinal cord, cerebellum, salivary gland, heart and colon. Isoform B is widely expressed in tissues and also in breast cancer and in prostate carcinoma cells. -
Sequence similarities
Belongs to the adaptor complexes large subunit family. -
Cellular localization
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. - Information by UniProt
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Database links
- Entrez Gene: 468956 Chimpanzee
- Entrez Gene: 100056103 Horse
- Entrez Gene: 160 Human
- Entrez Gene: 11771 Mouse
- Entrez Gene: 308578 Rat
- Omim: 601026 Human
- SwissProt: O95782 Human
- SwissProt: P17426 Mouse
see all -
Alternative names
- 100 kDa coated vesicle protein A antibody
- Adapter-related protein complex 2 alpha-1 subunit antibody
- adapter-related protein complex 2 subunit alpha-1 antibody
see all
Images
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Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.01 µg/ml + Human Frontal Cortex lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 108 kDa -
Lanes 1-3 : total protein blot stained with Ponceau Red
Lanes 4-6 : Anti-AP-2 complex subunit alpha-1 antibody (ab189995) at 0.1 µg/ml
Lanes 1 & 4 : Mouse Liver lysate
Lanes 2 & 5 : Mouse Brain lysate
Lanes 3 & 6 : Mouse Kidney lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 108 kDaThe left panel is stained with Ponceau red.
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Immunofluorescent analysis of methanol-fixed HeLa cells stably expressing a chimeric construct including the brain-specific splice variant of Mouse AP-2 complex subunit alpha-1 labeling AP-2 complex subunit alpha-1 with ab189995 at 0.1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling AP-2 complex subunit alpha-1 with ab189995 at 5µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab189995 has been referenced in 6 publications.
- Eichel K et al. Endocytosis in the axon initial segment maintains neuronal polarity. Nature 609:128-135 (2022). PubMed: 35978188
- Hutcheson JD et al. Elastogenesis Correlates With Pigment Production in Murine Aortic Valve Leaflets. Front Cardiovasc Med 8:678401 (2021). PubMed: 34239903
- Kell MJ et al. Novel function for AP-1B during cell migration. Mol Biol Cell 31:2475-2493 (2020). PubMed: 32816642
- Cai B et al. BMP2-mediated PTEN enhancement promotes differentiation of hair follicle stem cells by inducing autophagy. Exp Cell Res 385:111647 (2019). PubMed: 31562859
- Nie X et al. mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development. PLoS Genet 14:e1007491 (2018). PubMed: 29975682
- Bagh MB et al. Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model. Nat Commun 8:14612 (2017). IP, ICC/IF ; Mouse, Human . PubMed: 28266544