Overview

  • Product nameAnti-AP2 beta antibody
    See all AP2 beta primary antibodies
  • Description
    Rabbit polyclonal to AP2 beta
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: KQLCKEFTDL LAQDRTPIGN SRPSPILEPG IQSCLTHFSL ITHGFGAPAI, corresponding to C terminal amino acids 361-410 of Human AP2 beta

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
  • Involvement in diseaseDefects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
  • Sequence similaritiesBelongs to the AP-2 family.
  • Post-translational
    modifications
    Sumoylated on Lys-21; which inhibits transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 2 beta antibody
    • Activating enhancer-binding protein 2-beta antibody
    • AP 2B antibody
    • AP2 B antibody
    • AP2-beta antibody
    • AP2B antibody
    • AP2B_HUMAN antibody
    • AP2beta antibody
    • MGC21381 antibody
    • OTTHUMP00000039925 antibody
    • TFAP 2B antibody
    • Tfap2b antibody
    • Transcription factor AP 2 beta antibody
    • Transcription factor AP-2-beta antibody
    • Transcription factor AP2 beta antibody
    see all

Anti-AP2 beta antibody images

  • Anti-AP2 beta antibody (ab50906) at 1/2.5 dilution + Transfected 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-AP2 beta antibody (ab50906)

This product has been referenced in:
  • Williams MJ  et al. Obesity-linked homologues TfAP-2 and Twz establish meal frequency in Drosophila melanogaster. PLoS Genet 10:e1004499 (2014). Read more (PubMed: 25187989) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (neuroblastoma cell lines)
Loading amount 15 µg
Specification neuroblastoma cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"