Overview

  • Product nameAnti-AP2 beta antibody [AP2b 1F3/8]
    See all AP2 beta primary antibodies
  • Description
    Mouse monoclonal [AP2b 1F3/8] to AP2 beta
  • Tested applicationsWB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Truncated AP-2 beta-protein prepared from bacteria.

Properties

Applications

Our Abpromise guarantee covers the use of ab18113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent dilution.
IHC-Fr 1/300.

Target

  • FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
  • Involvement in diseaseDefects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
  • Sequence similaritiesBelongs to the AP-2 family.
  • Post-translational
    modifications
    Sumoylated on Lys-21; which inhibits transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 2 beta antibody
    • Activating enhancer-binding protein 2-beta antibody
    • AP 2B antibody
    • AP2 B antibody
    • AP2-beta antibody
    • AP2B antibody
    • AP2B_HUMAN antibody
    • AP2beta antibody
    • MGC21381 antibody
    • OTTHUMP00000039925 antibody
    • TFAP 2B antibody
    • TFAP2B antibody
    • Transcription factor AP 2 beta antibody
    • Transcription factor AP-2-beta antibody
    • Transcription factor AP2 beta antibody
    see all

Anti-AP2 beta antibody [AP2b 1F3/8] images

  • ab18113 at 1/300 staining perfusion fixed rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% + 15% of a saturated solution of picric acid in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/300) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000). The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The pictures show the staining obtained in cortical neurons, with the X20 (top picture) and X40 (bottom picture) objective.

    See Abreview

References for Anti-AP2 beta antibody [AP2b 1F3/8] (ab18113)

This product has been referenced in:
  • Bökenkamp R  et al. Dlx1 and Rgs5 in the ductus arteriosus: vessel-specific genes identified by transcriptional profiling of laser-capture microdissected endothelial and smooth muscle cells. PLoS One 9:e86892 (2014). IHC-P ; Rat . Read more (PubMed: 24489801) »
  • Ebauer M  et al. Comparative expression profiling identifies an in vivo target gene signature with TFAP2B as a mediator of the survival function of PAX3/FKHR. Oncogene 26:7267-81 (2007). WB ; Human . Read more (PubMed: 17525748) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat brain)
Specification Rat brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"