Anti-AP2 beta antibody [AP2b 1G6/2] (ab18114)

Overview

  • Product name
    Anti-AP2 beta antibody [AP2b 1G6/2]
    See all AP2 beta primary antibodies
  • Description
    Mouse monoclonal [AP2b 1G6/2] to AP2 beta
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Truncated AP-2 beta protein prepared from bacteria.

Properties

Applications

Our Abpromise guarantee covers the use of ab18114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-Fr
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-Fr: 1/1000. Use perfusion fixed tissue (see Abreview).
    WB: Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
    • Involvement in disease
      Defects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
    • Sequence similarities
      Belongs to the AP-2 family.
    • Post-translational
      modifications
      Sumoylated on Lys-21; which inhibits transcriptional activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating enhancer binding protein 2 beta antibody
      • Activating enhancer-binding protein 2-beta antibody
      • AP 2B antibody
      • AP2 B antibody
      • AP2-beta antibody
      • AP2B antibody
      • AP2B_HUMAN antibody
      • AP2beta antibody
      • MGC21381 antibody
      • OTTHUMP00000039925 antibody
      • PDA2 antibody
      • TFAP 2B antibody
      • Tfap2b antibody
      • Transcription factor AP 2 beta antibody
      • Transcription factor AP-2-beta antibody
      • Transcription factor AP2 beta antibody
      see all

    Anti-AP2 beta antibody [AP2b 1G6/2] images

    • ab18114 at 1/1000 staining perfusion fixed adult rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/1000) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000).

      The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The picture shows the staining obtained at the level of the spinal cord using the X40 objective.

      See Abreview

    References for Anti-AP2 beta antibody [AP2b 1G6/2] (ab18114)

    ab18114 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry.

    I am sorry to confirm that ab18114AP2 beta antibody [AP2b 1G6/2] has notbeen tested in IHC-P .

    It is likely that the antibody will still work in IHC-P, although we cannot provide our guarantee for this ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Adult rat brain)
    Specification
    Adult rat brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Jun 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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