Anti-AP2 gamma/TFAP2C antibody [3B5] (ab110635)
Key features and details
- Mouse monoclonal [3B5] to AP2 gamma/TFAP2C
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-AP2 gamma/TFAP2C antibody [3B5]
See all AP2 gamma/TFAP2C primary antibodies -
Description
Mouse monoclonal [3B5] to AP2 gamma/TFAP2C -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-7, A431, mouse placenta, and RAW 264.7 cell lysates; ICC/IF: HeLa, MCF-7, and U251 cells; IHC-P: Human breast cancer;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.85% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B5 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50.
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WB |
1/100 - 1/1000. Predicted molecular weight: 49 kDa.
Block with BSA only (not milk). |
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ICC/IF |
1/50 - 1/500.
|
Notes |
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IHC-P
1/50. |
WB
1/100 - 1/1000. Predicted molecular weight: 49 kDa. Block with BSA only (not milk). |
ICC/IF
1/50 - 1/500. |
Target
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Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. -
Sequence similarities
Belongs to the AP-2 family. -
Domain
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsSumoylated on Lys-10; which inhibits transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7022 Human
- Entrez Gene: 21420 Mouse
- Omim: 601602 Human
- SwissProt: Q92754 Human
- SwissProt: Q61312 Mouse
- Unigene: 473152 Human
- Unigene: 3629 Mouse
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Alternative names
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
see all
Images
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in Hela Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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All lanes : Anti-AP2 gamma/TFAP2C antibody [3B5] (ab110635) at 1/50 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Mouse placenta cell lysate
Lane 4 : RAW 264.7 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 49 kDa -
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with ab110635 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in U251 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in MCF-7 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab110635 has been referenced in 1 publication.
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139