Anti-AP2 gamma antibody (ab56030)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab56030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
  • Sequence similarities
    Belongs to the AP-2 family.
  • Domain
    The WW-binding motif mediates interaction with WWOX.
  • Post-translational
    modifications
    Sumoylated on Lys-10; which inhibits transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 2 gamma antibody
    • Activating enhancer-binding protein 2 gamma antibody
    • AP2-gamma antibody
    • AP2C_HUMAN antibody
    • AP2g antibody
    • AP2gamma antibody
    • ERF 1 antibody
    • ERF1 antibody
    • Estrogen receptor factor 1 antibody
    • hAP 2g antibody
    • hAP2g antibody
    • TFAP 2C antibody
    • TFAP 2G antibody
    • Tfap2c antibody
    • TFAP2G antibody
    • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
    • Transcription factor AP 2 gamma antibody
    • Transcription factor AP-2 gamma antibody
    • Transcription factor ERF 1 antibody
    • Transcription factor ERF-1 antibody
    • Transcription factor ERF1 antibody
    see all

Images



  • Predicted band size : 49 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling AP2 gamma with ab56030 at 1.0µg/ml.
  • Anti-AP2 gamma antibody (ab56030) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Gel concentration: 12%
  • ab56030, at 8 µg/ml, staining Human AP2 gamma in kidney by immunohistochemistry. Paraffin embedded tissue. Arrows indicate positively stained epithelial cells of the renal tubule (x400 magnification).

References

ab56030 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (Breast cancer cells)
Negative control
Non-specific primers to check the enrichment.
Specification
Breast cancer cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
ER-alpha ChIP using sc-543 antibody. Checking enrichment at known binding sites.
Username

Abcam user community

Verified customer

Submitted Sep 21 2016

Merci de votre intérêt pour les anti-AP2 gamma Abcam. Comme expliqué par téléphone, les anticorps ab31288, ab56030 et ab82788 n’ont pas encore été testé chez le rat et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cepen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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