Anti-AP2 gamma antibody [EP2692Y] (ab76007)

Overview

  • Product nameAnti-AP2 gamma antibody [EP2692Y]
    See all AP2 gamma primary antibodies
  • Description
    Rabbit monoclonal [EP2692Y] to AP2 gamma
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
    • Sequence similaritiesBelongs to the AP-2 family.
    • DomainThe WW-binding motif mediates interaction with WWOX.
    • Post-translational
      modifications
      Sumoylated on Lys-10; which inhibits transcriptional activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating enhancer binding protein 2 gamma antibody
      • Activating enhancer-binding protein 2 gamma antibody
      • AP2-gamma antibody
      • AP2C_HUMAN antibody
      • AP2g antibody
      • AP2gamma antibody
      • ERF 1 antibody
      • ERF1 antibody
      • Estrogen receptor factor 1 antibody
      • hAP 2g antibody
      • hAP2g antibody
      • TFAP 2C antibody
      • TFAP 2G antibody
      • Tfap2c antibody
      • TFAP2G antibody
      • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
      • Transcription factor AP 2 gamma antibody
      • Transcription factor AP-2 gamma antibody
      • Transcription factor ERF 1 antibody
      • Transcription factor ERF-1 antibody
      • Transcription factor ERF1 antibody
      see all

    Anti-AP2 gamma antibody [EP2692Y] images

    • All lanes : Anti-AP2 gamma antibody [EP2692Y] (ab76007) at 1/50000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : SKBR3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • ab76007 showing positive staining in Cervical carcinoma tissue.

    • ab76007 showing positive staining in Endometrial carcinoma tissue.

    • ab76007 showing positive staining in Prostatic carcinoma tissue.

    References for Anti-AP2 gamma antibody [EP2692Y] (ab76007)

    ab76007 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call today and for your question about ab76007.
    I've heard back from the lab, and this antibody is not predicted to cross react with AP2 alpha, beta, or epsilon. The exact immunogen shares 60%, 60%, and ˜0% identity with these A...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"