• Product nameAnti-AP2M1 antibody [EP2695Y]
    See all AP2M1 primary antibodies
  • Description
    Rabbit monoclonal [EP2695Y] to AP2M1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human AP2M1 aa 350 to the C-terminus (C terminal).
    Database link: Q96CW1

  • Positive control
    • WB: MCF-7 and SKBR-3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:
    Anti-AP2M1 antibody (HRP) [EP2695Y] (ab201772)
    Anti-AP2M1 antibody (Biotin) [EP2695Y] (ab201530)



Our Abpromise guarantee covers the use of ab75995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 50 kDa.

For unpurified use at 1/1000 - 1/5000.


  • FunctionComponent of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.
  • Sequence similaritiesBelongs to the adaptor complexes medium subunit family.
    Contains 1 MHD (mu homology) domain.
  • Cellular localizationCell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter-related protein complex 2 mu subunit antibody
    • Adaptin mu 1 antibody
    • Adaptin-mu2 antibody
    • Adaptor protein complex AP 2 subunit mu antibody
    • Adaptor protein complex AP-2 subunit mu antibody
    • Adaptor related protein complex 2 mu 1 subunit antibody
    • AP 2 mu 2 chain antibody
    • AP-2 complex subunit mu antibody
    • AP-2 mu chain antibody
    • Ap2m1 antibody
    • AP2M1_HUMAN antibody
    • AP50 antibody
    • CLAPM1 antibody
    • Clathrin adaptor complex AP2 mu subunit antibody
    • Clathrin assembly protein complex 2 medium chain antibody
    • Clathrin associated/assembly/adaptor protein medium 1 antibody
    • Clathrin coat adaptor protein AP50 antibody
    • Clathrin coat assembly protein AP50 antibody
    • Clathrin coat associated protein AP50 antibody
    • Clathrin coat-associated protein AP50 antibody
    • HA2 50 kDa subunit antibody
    • mu2 antibody
    • Plasma membrane adaptor AP-2 50 kDa protein antibody
    see all

Anti-AP2M1 antibody [EP2695Y] images

  • All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 50 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)

    Lane 1 : HEK293 cell lysate
    Lane 2 : SKBR-3 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 50 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution (unpurified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : SKBR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-AP2M1 antibody [EP2695Y] (ab75995)

This product has been referenced in:
  • Koller E  et al. Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytes. Nucleic Acids Res 39:4795-807 (2011). WB ; Mouse . Read more (PubMed: 21345934) »
  • Laulagnier K  et al. Role of AP1 and Gadkin in the traffic of secretory endo-lysosomes. Mol Biol Cell 22:2068-82 (2011). Read more (PubMed: 21525240) »

See all 2 Publications for this product

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Merci de nous avoir contactés.

Anti-AP2M1(ab75995) a été testé sur des lysats de cellules MCF-7 et SKBR-3. Anti- CTLA4 (ab134090) a été testé sur des lysats de cellules HELA, Raji, MOLT4 et K56...

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