Recombinant Anti-AP2M1 antibody [EP2695Y] (ab75995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2695Y] to AP2M1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AP2M1 antibody [EP2695Y]
See all AP2M1 primary antibodies -
Description
Rabbit monoclonal [EP2695Y] to AP2M1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human AP2M1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96CW1 -
Positive control
- WB: MCF-7, HeLa and SKBR-3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2695Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/10000. Predicted molecular weight: 50 kDa.
For unpurified use at 1/1000 - 1/5000. |
Notes |
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WB
1/10000. Predicted molecular weight: 50 kDa. For unpurified use at 1/1000 - 1/5000. |
Target
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Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling. -
Sequence similarities
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
Cellular localization
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. - Information by UniProt
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Database links
- Entrez Gene: 1173 Human
- Entrez Gene: 11773 Mouse
- Entrez Gene: 116563 Rat
- Omim: 601024 Human
- SwissProt: Q96CW1 Human
- SwissProt: P84091 Mouse
- SwissProt: P84092 Rat
- Unigene: 518460 Human
see all -
Alternative names
- Adapter-related protein complex 2 mu subunit antibody
- Adaptin mu 1 antibody
- Adaptin-mu2 antibody
see all
Images
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All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDa
Exposure time: 7 secondsWe recommend not to boil the samples after lysis to get desired WB results.
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)
Lane 1 : Rat brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)
Lane 1 : HEK293 cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab75995 has been referenced in 19 publications.
- Puray-Chavez M et al. Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. Cell Rep 36:109364 (2021). PubMed: 34214467
- Puray-Chavez M et al. Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. bioRxiv N/A:N/A (2021). PubMed: 33688646
- Yasumura M et al. Planar cell polarity protein Vangl2 and its interacting protein Ap2m1 regulate dendritic branching in cortical neurons. Genes Cells 26:987-998 (2021). PubMed: 34626136
- Tongmuang N et al. Suppression of µ1 subunit of the adaptor protein complex 2 reduces dengue virus release. Virus Genes 56:27-36 (2020). PubMed: 31720911
- Heaton GR et al. Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis. Neurobiol Dis 141:104948 (2020). PubMed: 32434048