Overview

  • Product name
  • Description
    Rabbit polyclonal to AP2S1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human AP2S1 aa 35-64 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P53680

  • Positive control
    • Hela cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.

Target

  • Function
    Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
  • Sequence similarities
    Belongs to the adaptor complexes small subunit family.
  • Cellular localization
    Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter-related protein complex 2 sigma subunit antibody
    • Adaptor protein complex AP 2 subunit sigma antibody
    • Adaptor protein complex AP-2 subunit sigma antibody
    • Adaptor protein complex AP2 subunit sigma antibody
    • Adaptor related protein complex 2 sigma 1 subunit antibody
    • AI043088 antibody
    • AP 17 antibody
    • AP 2 complex subunit sigma 1 antibody
    • AP-2 complex subunit sigma antibody
    • AP17 antibody
    • AP17 delta antibody
    • AP2 complex subunit sigma 1 antibody
    • Ap2s1 antibody
    • AP2S1_HUMAN antibody
    • CLAPS 2 antibody
    • CLAPS2 antibody
    • Clathrin adaptor complex AP2, sigma subunit antibody
    • Clathrin adaptor protein AP17 antibody
    • Clathrin assembly protein 2 small chain antibody
    • Clathrin associated/assembly/adaptor protein small 2 antibody
    • Clathrin associated/assembly/adaptor protein small 2, 17-KD antibody
    • Clathrin associated/assembly/adaptor protein, small 2 (17kD) antibody
    • Clathrin coat assembly protein AP17 antibody
    • Clathrin coat associated protein AP17 antibody
    • Clathrin coat-associated protein AP17 antibody
    • HA2 17 kDa subunit antibody
    • MGC62945 antibody
    • Plasma membrane adaptor AP 2 17 kDa protein antibody
    • Plasma membrane adaptor AP-2 17 kDa protein antibody
    • Plasma membrane adaptor AP2 17 kDa protein antibody
    • Sigma adaptin 3b antibody
    • Sigma2 adaptin antibody
    • Sigma2-adaptin antibody
    see all

Images

  • Anti-AP2S1 antibody (ab173201) at 1/100 dilution + Hela cell line lysates at 35 µg

    Predicted band size : 17 kDa

References

ab173201 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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