Overview

  • Product name
  • Description
    Goat polyclonal to AP3
  • Specificity
    Recognises a strong band at 130kD representing AP3. Also detects a band at about 80kD probably representing non-specific binding.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SPSPQKRYQDTPGC

    , corresponding to amino acids 686-698 of Human AP3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

Target

  • Function
    Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
  • Tissue specificity
    Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.
  • Sequence similarities
    Belongs to the adaptor complexes large subunit family.
    Contains 11 HEAT repeats.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter-related protein complex 3 subunit delta-1 antibody
    • adaptor-related protein complex 3, delta 1 subunit antibody
    • ADTD antibody
    • AP 3 complex delta subunit antibody
    • AP-3 complex subunit delta antibody
    • AP-3 complex subunit delta-1 antibody
    • ap3d1 antibody
    • AP3D1_HUMAN antibody
    • Delta 1 subunit of adaptor-related protein complex 3 antibody
    • Delta Adaptin antibody
    • Delta-adaptin antibody
    • hBLVR antibody
    • Subunit of putative vesicle coat adaptor complex AP 3 antibody
    see all

Images



  • Predicted band size : 130 kDa


    Western blot using ab3705 on HeLa whole cell extract at 1/500.

    Secondary ab: Rabbit polyclonal to Goat IgG (HRP) ab6741 (1/5000)
    Exposure time: 30 seconds.
    Lysate at 20µg/lane.

    Predicted molecular weight:130 kDa. The band at about 80kD probably represents non-specific binding.

References

ab3705 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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