Overview

  • Product nameAnti-AP3B2 antibody
    See all AP3B2 primary antibodies
  • Description
    Rabbit polyclonal to AP3B2
  • SpecificityAt least three isoforms of AP3B2 are known to exist; this antibody will recognize the two larger isoforms.
  • Tested applicationsSuitable for: WB, ICC/IF, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid synthetic peptide from near the amino terminus of Human AP3B2 (NP_004635).

  • Positive control
    • Rat brain tissue. Mouse brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab113100 is purified by affinity chromatography via a peptide column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab113100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 119 kDa.
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionSubunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles.
  • Tissue specificityExpression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression).
  • Sequence similaritiesBelongs to the adaptor complexes large subunit family.
  • Developmental stageExpressed from early in development through adulthood.
  • Cellular localizationGolgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter-related protein complex 3 subunit beta-2 antibody
    • Adaptor protein complex AP-3 subunit beta-2 antibody
    • Adaptor related protein complex 3 beta 2 subunit antibody
    • AP 3 complex subunit beta 2 antibody
    • AP-3 complex subunit beta-2 antibody
    • AP3B2 antibody
    • AP3B2_HUMAN antibody
    • beta NAP antibody
    • Beta-3B-adaptin antibody
    • Beta3B adaptin antibody
    • Beta3b antibody
    • Clathrin assembly protein complex 3 beta 2 large chain antibody
    • Clathrin assembly protein complex 3 beta-2 large chain antibody
    • NAPTB antibody
    • Neuron specific vesicle coat protein and cerebellar degeneration antigen antibody
    • Neuron-specific vesicle coat protein beta-NAP antibody
    • Neuronal adaptin like protein beta subunit antibody
    see all

Anti-AP3B2 antibody images

  • Immunofluorescence of AP3B2 in mouse brain tissue with ab113100 at 20 ug/mL.
  • Lane 1 : Anti-AP3B2 antibody (ab113100) at 1 µg/ml
    Lane 2 : Anti-AP3B2 antibody (ab113100) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 119 kDa
  • Immunohistochemical analysis of mouse brain tissue, staining AP3B2 with ab113100 at 5 µg/ml.

References for Anti-AP3B2 antibody (ab113100)

ab113100 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"