The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPart of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Tissue specificityPresent in all adult tissues examined.
Sequence similaritiesBelongs to the adaptor complexes small subunit family.
Cellular localizationGolgi apparatus. Cytoplasmic vesicle membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.