Overview

  • Product nameAnti-AP4 antibody
    See all AP4 primary antibodies
  • Description
    Rabbit polyclonal to AP4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: GNCGRSFAQW DQLVAHKRVH VAEALEEAAA KALGPRPRGR PAVTAPRPGG , corresponding to a region within internal sequence amino acids 180 - 229 of Human AP4 (NP_037532)

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • RelevanceAP4 (Replication initiator 1) is a sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. It binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. AP4 facilitates DNA bending.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 60 kDa origin specific DNA binding protein antibody
    • 60 kDa replication initiation region protein antibody
    • ATT binding protein antibody
    • DHFR oribeta binding protein RIP60 antibody
    • H_DJ0584D14.12 antibody
    • REPIN1 antibody
    • Replication initiation region protein (60kD) antibody
    • Replication initiator 1 antibody
    • RIP60 antibody
    • Zfp464 antibody
    • Zinc finger protein 464 (RIP60) antibody
    • Zinc finger proten AP4 antibody
    • ZNF464 antibody
    see all

Anti-AP4 antibody images

  • Anti-AP4 antibody (ab83414) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-AP4 antibody (ab83414)

ab83414 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"