Anti-AP4M1 antibody (ab96306)
Key features and details
- Rabbit polyclonal to AP4M1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP4M1 antibody -
Description
Rabbit polyclonal to AP4M1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within the internal sequence amino acids 70-265 of Human AP4M1.
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Positive control
- 293T, A431, Jurkat, Raji cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
Target
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Function
Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. -
Tissue specificity
Ubiquitous. Highly expressed in testis and lowly expressed in brain and lung. -
Involvement in disease
Defects in AP4M1 are the cause of cerebral palsy spastic quadriplegic type 3 (CPSQ3) [MIM:612936]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals present postnatally with early infantile hypotonia, delayed psychomotor development, strabismus, lack of independent walking and severe mental retardation. They develop progressive spasticity of all limbs with generalized hypertonia, hyperreflexia, and extensor plantar responses by the end of the first year of life. Speech is absent or limited. Pseudobulbar signs, such as drooling, stereotypic laughter, and exaggerated jaw jerk, are part of the clinical picture. -
Sequence similarities
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
Domain
Interacts specifically with tyrosine-based sorting signals. -
Cellular localization
Golgi apparatus > trans-Golgi network. Membrane > coated pit. Associated with the trans-Golgi network. Found in soma and dendritic shafts of neuronal cells. - Information by UniProt
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Database links
- Entrez Gene: 768040 Cow
- Entrez Gene: 9179 Human
- Entrez Gene: 11781 Mouse
- Entrez Gene: 304344 Rat
- Omim: 602296 Human
- SwissProt: Q29RY8 Cow
- SwissProt: O00189 Human
- SwissProt: Q9JKC7 Mouse
see all -
Alternative names
- Adapter-related protein complex 4 mu-1 subunit antibody
- Adaptor related protein complex 4 mu 1 subunit antibody
- Adaptor related protein complex AP 4 mu4 subunit antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab96306 has been referenced in 2 publications.
- Contu VR et al. Lysosomal targeting of SIDT2 via multiple YxxF motifs is required for SIDT2 function in the process of RNautophagy. J Cell Sci 130:2843-2853 (2017). PubMed: 28724756
- Toh WH et al. Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b- and AP4-dependent pathway. Traffic 18:159-175 (2017). PubMed: 28000370