Overview

  • Product name
  • Description
    Rabbit polyclonal to AP4S1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LDEYFSRVSE LDVSFFNTVF HSTWQMHSGP YQEPIDELPK ICSALEPQQT CFSPDSSSFK GAASTT, corresponding to amino acids 91-156 of Human AP4S1, isoform 2.

  • Positive control
    • Human Rectum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the adaptor complexes small subunit family.
  • Cellular localization
    Golgi apparatus > trans-Golgi network. Membrane > coated pit. Associated with the trans-Golgi network. Found in soma and dendritic shafts of neuronal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter-related protein complex 4 subunit sigma-1 antibody
    • AP-4 adapter complex subunit sigma-1 antibody
    • AP-4 complex subunit sigma-1 antibody
    • AP47B antibody
    • Ap4s1 antibody
    • AP4S1_HUMAN antibody
    • CLA20 antibody
    • CLAPS4 antibody
    • CPSQ6 antibody
    • Sigma-1 subunit of AP-4 antibody
    • Sigma-4-adaptin antibody
    • Sigma4-adaptin antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122607 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122607, at 1/50 dilution, staining AP4S1 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry.

References

ab122607 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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