Overview

  • Product nameAnti-APAF1 antibody [2E10]
    See all APAF1 primary antibodies
  • Description
    Mouse monoclonal [2E10] to APAF1
  • Tested applicationsSuitable for: WB, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids near the carboxy terminus of human Apaf1.

  • Positive control
    • K562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC (PFA fixed)
  • Application notesImmunohistochemistry (PFA fixed): Use at a concentration of 0.5 µg/ml.
    WB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 141 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
    • Tissue specificityUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
    • Sequence similaritiesContains 1 CARD domain.
      Contains 1 NB-ARC domain.
      Contains 13 WD repeats.
    • DomainThe CARD domain mediates interaction with APIP.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF 1 antibody
      • Apaf-1 antibody
      • APAF_HUMAN antibody
      • Apaf1 antibody
      • Apoptotic peptidase activating factor 1 antibody
      • Apoptotic protease activating factor 1 antibody
      • Apoptotic protease activating factor antibody
      • Apoptotic protease-activating factor 1 antibody
      • CED 4 antibody
      • CED4 antibody
      • KIAA0413 antibody
      see all

    Anti-APAF1 antibody [2E10] images

    • Lane 1 : Anti-APAF1 antibody [2E10] (ab36822) at 0.25 µg/ml
      Lane 2 : Anti-APAF1 antibody [2E10] (ab36822) at 0.5 µg/ml

      Lane 1 : K562 cell lysate
      Lane 2 : K562 cell lysate


      Predicted band size : 141 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    • ab36822 at 0.5µg/ml staining APAF1 in K562 cells by IHC-P

    References for Anti-APAF1 antibody [2E10] (ab36822)

    ab36822 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab36822.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"