Overview

  • Product nameAnti-APAF1 antibody [5E11]
    See all APAF1 primary antibodies
  • Description
    Mouse monoclonal [5E11] to APAF1
  • Tested applicationsSuitable for: IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids near the carboxy terminus of human APAF1.

  • Positive control
    • K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab36824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
  • Application notesImmunohistochemistry (PFA fixed): Use at a concentration of 0.5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
    • Tissue specificityUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
    • Sequence similaritiesContains 1 CARD domain.
      Contains 1 NB-ARC domain.
      Contains 13 WD repeats.
    • DomainThe CARD domain mediates interaction with APIP.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF 1 antibody
      • Apaf-1 antibody
      • APAF_HUMAN antibody
      • Apaf1 antibody
      • Apoptotic peptidase activating factor 1 antibody
      • Apoptotic protease activating factor 1 antibody
      • Apoptotic protease activating factor antibody
      • Apoptotic protease-activating factor 1 antibody
      • CED 4 antibody
      • CED4 antibody
      • KIAA0413 antibody
      see all

    Anti-APAF1 antibody [5E11] images

    • ab36824 at 0.5µg/ml staining APAF1 in K562 cells by IHC

    References for Anti-APAF1 antibody [5E11] (ab36824)

    ab36824 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab36824.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"