Anti-APAF1 antibody (ab2000)

Overview

  • Product name
  • Description
    Rabbit polyclonal to APAF1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).
    Sequence:

    TFYTNGTNLKKIHVSPDFKT


    (Peptide available as ab7872)

  • Positive control
    • Human heart tissue lysate IF/ICC: HEK293 cell line
  • General notes
    Apoptosis Protease Activating Factor

Properties

Applications

Our Abpromise guarantee covers the use of ab2000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 20 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.Can be blocked with APAF1 peptide (ab7872).

Target

  • Function
    Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • Tissue specificity
    Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • Sequence similarities
    Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • Domain
    The CARD domain mediates interaction with APIP.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

Anti-APAF1 antibody images

  • ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml

    Lane 1 : Human heart tissue lysate with absence of blocking peptide
    Lane 2 : Human heart tissue lysate with presence of blocking peptide


    Predicted band size : 130 kDa
    Observed band size : 130 kDa
  • Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.

References for Anti-APAF1 antibody (ab2000)

ab2000 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (Citrate)
Permeabilization
No
Specification
Lymph node
Fixative
Paraformaldehyde
Username

Lina Li

Verified customer

Submitted Jun 05 2017

Thank you for your reply.

I have processed your request to receive the 2 free of charge replacements and below is the testing discount code for ab93942.

Please let me know if there is anything else I can help you with.

DIS...

Read More

Thank you for your reply.

I am sorry that the customer has had problems with these antibodies. As we guarantee thatboth ab2000 and ab32351 to work in western blot on rat tissue and they are not working as we expect then I am happy to either r...

Read More

Thank you for contacting Abcam.

I am sorry about the problems that you have been having in western blot, with some of our products. Based on the information you have provided, I suspect that the problem is with the secondary antibody, since i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (C57Bl/6 mouse liver)
Loading amount
10 µg
Specification
C57Bl/6 mouse liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 19 2011

Anti-Apaf-1 NT (2013) and anti-Apaf-1 CT (2015) were gernerated with peptides selected from the N-terminus and C-terminus of the protein. 888 513 9525

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up