Overview

  • Product nameAnti-APAF1 antibody
    See all APAF1 primary antibodies
  • Description
    Rabbit polyclonal to APAF1
  • Tested applicationsSuitable for: IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from C terminal of Apaf-1(Human)

  • Positive control
    • Testis

Properties

Applications

Our Abpromise guarantee covers the use of ab46899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
  • Application notesIHC-Fo: 1/200.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM
    Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
    • Tissue specificityUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
    • Sequence similaritiesContains 1 CARD domain.
      Contains 1 NB-ARC domain.
      Contains 13 WD repeats.
    • DomainThe CARD domain mediates interaction with APIP.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF 1 antibody
      • Apaf-1 antibody
      • APAF_HUMAN antibody
      • Apaf1 antibody
      • Apoptotic peptidase activating factor 1 antibody
      • Apoptotic protease activating factor 1 antibody
      • Apoptotic protease activating factor antibody
      • Apoptotic protease-activating factor 1 antibody
      • CED 4 antibody
      • CED4 antibody
      • KIAA0413 antibody
      see all

    Anti-APAF1 antibody images

    • Human testis stained with ab46899 using the antibody at a dilution of 1:200

    References for Anti-APAF1 antibody (ab46899)

    ab46899 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab46899.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"