Anti-APAF1 antibody, prediluted (ab15178)

Overview

  • Product nameAnti-APAF1 antibody, prediluted
    See all APAF1 primary antibodies
  • Description
    Rabbit polyclonal to APAF1, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • Tested with testis cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready to use. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
    • Tissue specificityUbiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
    • Sequence similaritiesContains 1 CARD domain.
      Contains 1 NB-ARC domain.
      Contains 13 WD repeats.
    • DomainThe CARD domain mediates interaction with APIP.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF 1 antibody
      • Apaf-1 antibody
      • APAF_HUMAN antibody
      • Apaf1 antibody
      • Apoptotic peptidase activating factor 1 antibody
      • Apoptotic protease activating factor 1 antibody
      • Apoptotic protease activating factor antibody
      • Apoptotic protease-activating factor 1 antibody
      • CED 4 antibody
      • CED4 antibody
      • KIAA0413 antibody
      see all

    Anti-APAF1 antibody, prediluted images

    • Human testis stained with ab15178.

    References for Anti-APAF1 antibody, prediluted (ab15178)

    ab15178 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. Further to correspondence with my colleague I can tell you that the undiluted version of ab15178 has the catalogue code ab46899. The antibody datasheet will be available from Monday morning. You can place your order in t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"