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Our Abpromise guarantee covers the use of ab3448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 93 kDa).Can be blocked with APBA1 peptide (ab4980).
By Western blot, this antibody detects an ~130 kDa protein representing Mint 1 from rat forebrain postnuclear supernatant.
Western blot of APBA1 on extract from HEK293 cells overexpressing the rat gene using ab3448.