• Product nameAnti-APC antibody
    See all APC primary antibodies
  • Description
    Rabbit polyclonal to APC
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal)

  • Positive control
    • Normal colon tissue. A431 cell line.



Our Abpromise guarantee covers the use of ab15270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 312 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionTumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
  • Tissue specificityExpressed in a variety of tissues.
  • Involvement in diseaseDefects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years.
    Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also known as familial infiltrative fibromatosis (FIF). HDD is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis.
    Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome).
    Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
    Defects in APC are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
    Defects in APC are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. This defect includes also the disease entity termed hepatoblastoma.
  • Sequence similaritiesBelongs to the adenomatous polyposis coli (APC) family.
    Contains 7 ARM repeats.
  • DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    Phosphorylated by GSK3B.
    Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
  • Cellular localizationCell junction > adherens junction. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle membrane. Cytoplasm. Cell membrane. Associated with the microtubule network at the growing distal tip of microtubules. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenomatous Polyposis Coli antibody
    • Adenomatous polyposis coli protein antibody
    • Apc antibody
    • APC_HUMAN antibody
    • CC1 antibody
    • Deleted in polyposis 2.5 antibody
    • DP2 antibody
    • DP2.5 antibody
    • DP3 antibody
    • FAP antibody
    • FPC antibody
    • GS antibody
    • Protein APC antibody
    see all

Anti-APC antibody images

  • ICC/IF image of ab15270 stained A431 cells. The cells were 4% paraformalehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Normal human colon section stained with ab15270 (1:100 for 10 min at RT). Sections were pre-treated by boiling in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

References for Anti-APC antibody (ab15270)

This product has been referenced in:
  • Shu Z  et al. miR-582-5P induces colorectal cancer cell proliferation by targeting adenomatous polyposis coli. World J Surg Oncol 14:239 (2016). WB ; Human . Read more (PubMed: 27595705) »
  • Liu R  et al. Fstl1 is involved in the regulation of radial glial scaffold development. Mol Brain 8:53 (2015). IF . Read more (PubMed: 26382033) »

See all 10 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Liver and heart)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Liver and heart
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (colonic epithelial cells)
Specification colonic epithelial cells
Permeabilization Yes - Triton 0.01%
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Nov 25 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mammary tumor)
Specification mammary tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Xenopus laevis Cell lysate - other (Egg)
Total protein in input 625 µg
Specification Egg
Immuno-precipitation step Other - Dynabeads

Abcam user community

Verified customer

Submitted Feb 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK)
Loading amount 20 µg
Specification HEK
Gel Running Conditions Non-reduced Denaturing (7.5% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Egg)
Loading amount 25 µg
Specification Egg
Gel Running Conditions Reduced Denaturing (7%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Elsie Hodimont

Verified customer

Submitted Aug 03 2012

Je suis ravie que vous souhaitiez participer a notre offre et tester ab15270 chez le xénope. Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un ...

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Merci de votre intérêt pour nos anticorps.

Malheureusement aucun de nos anticorps contre le APC ont déjà été testé contre le Xénope. J'ai vérifié avec le laboratoire, et ils...

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Thank you for contacting us.

ab15270 should only detect the full length APC protein.

I hope the information is useful to you. Please do not hesitate to contact me if you have further questions.

As requested, I have issued a free of charge replacement with the order number 996071 (one unit of ab15270). To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge...

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