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Read our guarantee »Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Anti-APC antibody
See all APC products (11) ...
Rabbit polyclonal to APC
ICC/IF, WB, IP, IHC-Pmore details
Reacts with
Mouse, Human
Predicted to work with
Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal)
Normal colon tissue.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - APC antibody (ab15270)
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Our Abpromise guarantee covers the use of ab15270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21402068)
WB: Use at an assay dependent dilution. Predicted molecular weight: 312 kDa.
IP: Use at an assay dependent dilution.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Cell junction; adherens junction
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - APC antibody (ab15270)

Normal human colon section stained with ab15270 (1:100 for 10 min at RT). Sections were pre-treated by boiling in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab15270? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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