APC antibody (ab15270)

Product Name 

APC antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to APC

Immunogen 

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 312 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Normal colon tissue.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

Relevance 

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration.
Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C.

See below for APC antibody images, references, products related to ab15270 and other tools.

APC antibody images:

References for APC antibody (ab15270)

  This product has been used in:

Ivaniutsin U et al. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev 4:3 (2009). ICC/IF; Mouse. PubMed: 19149881

If you publish research using ab15270 please let us know so that we can cite the reference on this datasheet.

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