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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-APC antibody
See all APC products (11) ...
Description
Rabbit polyclonal to APC
Tested applications
ICC/IF, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human)(C terminal)
Positive control
Normal colon tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Cancer >> Cell cycle >> Cell division
Stem Cells >> Signaling Pathways >> Wnt >> Cytoplasmic
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - APC antibody (ab15270)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21402068)
WB: Use at an assay dependent dilution. Predicted molecular weight: 312 kDa.
IP: Use at an assay dependent dilution.
IHC-P: 1/100Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Relevance
Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Cellular localization
Cell junction; adherens junction
Alternative names
- Adenomatous Polyposis Coli antibody
- APC antibody
- CC1 antibody
see all
Database links
- Entrez Gene: 324 Human
- Entrez Gene: 11789 Mouse
- Entrez Gene: 24205 Rat
- Omim: 175100 Human
- SwissProt: P25054 Human
- SwissProt: Q61315 Mouse
- SwissProt: P70478 Rat
- Unigene: 88057 Rat
Anti-APC antibody images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - APC antibody (ab15270)
Normal human colon section stained with ab15270 (1:100 for 10 min at RT). Sections were pre-treated by boiling in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
References for Anti-APC antibody (ab15270)
This product has been referenced in:
- Miclea RLet al. Apc bridges Wnt/ß-catenin and BMP signaling during osteoblast differentiation of KS483 cells. Exp Cell Res 317:1411-21 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 21402068) »
- Ivaniutsin Uet al. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev 4:3 (2009). ICC/IF; Mouse.Read more (PubMed: 19149881) »
See all 2 publications for this product
Publishing research using ab15270? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"