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Amino acids 1-433 N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) fused to maltose binding protein.
Our Abpromise guarantee covers the use of ab58 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23658710|
|Flow Cyt||Use 1µg for 106 cells.|
|WB||Use at an assay dependent concentration. This antibody has been used routinely for Western blotting on colon cell line HCT116, SW480 and SW837 extracts.|
|IHC-Fr||Use at an assay dependent concentration.|
Overlay histogram showing HCT116 cells stained with ab58 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.