The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
Use at an assay dependent dilution.
FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the APC1 family. Contains 4 PC repeats.
Post-translational modificationsPhosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
References for Anti-Apc1 (phospho S377) antibody (ab12206)
has not yet been referenced specifically in any publications.
Publishing research using ab12206? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"