Anti-Apc1 (phospho S377) antibody (ab12206)

Overview

  • Product name
    Anti-Apc1 (phospho S377) antibody
    See all Apc1 primary antibodies
  • Description
    Rabbit polyclonal to Apc1 (phospho S377)
  • Specificity
    ab12206 is specific for human APC1 phosphorylated at S377. Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: C-SHNQSpPKRHS, corresponding to amino acids 373-382 of Human Apc1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC1 family.
    Contains 4 PC repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC 1 antibody
    • Anapc1 antibody
    • Anaphase promoting complex subunit 1 antibody
    • anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody
    • Anaphase-promoting complex subunit 1 antibody
    • Apc 1 antibody
    • APC1 antibody
    • APC1_HUMAN antibody
    • Cyclosome subunit 1 antibody
    • MCPR antibody
    • Meiotic checkpoint regulator antibody
    • Mitotic checkpoint regulator antibody
    • Protein Tsg 24 antibody
    • Protein Tsg24 antibody
    • Testis-specific gene 24 protein antibody
    • TSG 24 antibody
    • TSG24 antibody
    see all

Images

  • All lanes : Anti-Apc1 (phospho S377) antibody (ab12206) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate from asynchoronous cells
    Lane 2 : HeLa whole cell lysate from cells treated with nocodazole

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat-anti-Rabbit IgG [H&L] MX at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 215 kDa
    Observed band size : 215 kDa
  • ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X.  This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

References

ab12206 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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