Overview

  • Product name
  • Description
    Rabbit polyclonal to Apc10
  • Specificity
    This antibody detects a band at about 26kD that corresponds to Apc10.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    GADPKQLERTG

    , corresponding to amino acids 10-20 of Human Apc10.

  • General notes
    Expiration date is one (1) year from date of opening.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC10 family.
    Contains 1 DOC domain.
  • Information by UniProt
  • Database links
  • Form
    It localises to Cytosol and nucleoplasm.
  • Alternative names
    • ANAPC 10 antibody
    • anapc10 antibody
    • Anaphase promoting complex 10 antibody
    • Anaphase promoting complex subunit 10 antibody
    • Anaphase-promoting complex subunit 10 antibody
    • Apc 10 antibody
    • APC10 antibody
    • APC10_HUMAN antibody
    • Cyclosome subunit 10 antibody
    • DKFZP564L0562 antibody
    • Doc 1 antibody
    • Doc1 antibody
    • Doc1, S. cerevisiae, homolog of antibody
    • OTTHUMP00000220297 antibody
    • OTTHUMP00000220298 antibody
    • OTTHUMP00000220299 antibody
    • OTTHUMP00000220300 antibody
    • OTTHUMP00000220303 antibody
    see all

Images

  • All lanes : Anti-Apc10 antibody (ab3629) at 1/500 dilution

    Lane 1 : Hela nuclear lysates
    Lane 2 : Hela cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size : 21 kDa


    Exposure time : 4 minutes

References

ab3629 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab3629.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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