Overview

  • Product name
    Anti-APC16 antibody
  • Description
    Rabbit polyclonal to APC16
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: VSGSSVTGSG FSVSDLAPPR KALFTYPKGA GEMLEDGSER FLCESVFSYQ VASTLKQVKH DQQVARMEKL AGLVEELEAD EWRFKPIEQ, corresponding to amino acids 13-101 of Human APC16.

  • Positive control
    • RT4, U-251 MG, Human Plasma and Human Liver lysates, Human colon tissue, U-2 OS cells

Properties

Applications

Our Abpromise guarantee covers the use of ab122416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC16 family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • anapc16 antibody
    • Anaphase-promoting complex subunit 16 antibody
    • APC16 antibody
    • APC16_HUMAN antibody
    • bA570G20.3 antibody
    • C10orf104 antibody
    • CENP-27 antibody
    • centromere protein 27 antibody
    • Chromosome 10 open reading frame 104 antibody
    • Cyclosome subunit 16 antibody
    • metabolic syndrome-associated gene antibody
    • metabolic syndrome-associated protein antibody
    • MSAG antibody
    see all

Images

  • ab122416 at 1/150 dilution staining APC16 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line U-2 OS using ab122416 at a concentration of 4 µg/ml. ab122416 staining is shown in green.

References

ab122416 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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