Overview

  • Product nameAnti-APC2 antibody
    See all APC2 primary antibodies
  • Description
    Rabbit polyclonal to APC2
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human APC2 (NP_005874.1).

  • Positive control
    • Human Brain Cortex and Small Intestine tissues; HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab113370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 244 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC Use at an assay dependent concentration.

Target

  • RelevanceAPC2 might function in the Wnt signaling pathway through it's ability to promote the rapid degradation of beta-catenin. It also functions as a tumour suppressor.
  • Cellular localizationCell Membrane, Cytoplasmic and Golgi Apparatus
  • Database links
  • Alternative names
    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APCL antibody

Anti-APC2 antibody images

  • ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry.
  • ab113370, at 5 µg/ml, staining APC2 in Formalin-fixed, Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.
  • Lane 1 : Anti-APC2 antibody (ab113370) at 1 µg/ml
    Lane 2 : Anti-APC2 antibody (ab113370) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 244 kDa

References for Anti-APC2 antibody (ab113370)

This product has been referenced in:
  • Hebert AM  et al. Merlin/ERM proteins establish cortical asymmetry and centrosome position. Genes Dev 26:2709-23 (2012). WB ; Human . Read more (PubMed: 23249734) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small bowel)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization No
Specification Small bowel
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde
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Submitted Sep 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"