Overview

  • Product nameAnti-Apc2 antibodySee all Apc2 primary antibodies ...
  • Description
    Mouse monoclonal to Apc2
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 716-823 of Human Apc2

Properties

Applications

Our Abpromise guarantee covers the use of ab57094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the cullin family.
    • Target information above from: UniProt accession Q9UJX6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • anapc2 antibody
      • Anaphase promoting complex subunit 2 antibody
      • Anaphase-promoting complex subunit 2 antibody
      • ANC2_HUMAN antibody
      • APC2 antibody
      • Cyclosome subunit 2 antibody
      • KIAA1406 antibody
      • OTTHUMP00000022692 antibody
      • RP11 350O14.5 antibody
      see all

    Anti-Apc2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57094 Apc2 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

    References for Anti-Apc2 antibody (ab57094)

    ab57094 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (U20S, RPE1, HELA)
    Loading amount 50 µg
    Specification U20S, RPE1, HELA
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Username

    Dr. Rob Wolthuis

    Verified customer

    Submitted Feb 24 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"